DBT One Health Consortium

Output - Release of Roll AMP ASFV Kit

A rapid, simple, and sensitive diagnostic technique for the detection of African swine fever virus (ASFV) nucleic acid was developed for testing clinical samples in the field or resource-constrained settings. The saltatory rolling-circle amplification (SRCA) technique was used for the first time to detect ASFV. The technique was developed using World Organization for Animal Health (WOAH)-approved primers targeting the p72 gene of the ASFV genome. The assay can be performed within 90 minutes at an isothermal temperature of 58°C without a requirement for sophisticated instrumentation. The same was released by Shri Narendra Singh Tomar, Union Minister of Agriculture during the Krishi Kumbh, 2023, held at ICAR RC NEH, Umiam during 4-6th January 2023.